Personal Homepage

University of Graz


Faculty of Natural Sciences


Institute Structure


Staff Members


Teaching


Information


Pharma-Links


Home

Administrator* 20.6.05
Prof. Dr. Andreas Kungl Ao. Univ.-Prof. Dr. Andreas Kungl

Building: Universitätsplatz 1, Room 111
A-8010 Graz / Austria

Tel: +43-316-380-5373 (Office)
Fax: +43-316-382541

e-mail: Andreas.Kungl@uni-graz.at




Research focus on:

  • Use of molecular biological techniques to create high-yield bacterial and eukaryotic protein expression systems for structure determination
  • Cell biology for the establishment of stable-transfected cell lines overexpressing recombinant proteins
  • Generation of site-directed mutants to study the role of certain amino acids with respect to the function (= ligand binding) of the protein
  • Protein chemistry: expression, purification and characterisation of recombinant proteins
  • Spectroscopy (fluorescence, circular dichroism, NMR) and microcalorimetry of proteins to investigate protein structure and ligand interactions, ligands include carbohydrates, nucleic acids, and synthetic (lead) compounds
  • Proteomics: pharmaceutical target profiling (protein functional analysis) by 2-D gel electrophoresis, multidimensional chromatography and LC-MS/MS sequence analysis

 

Research Topics:

  • Protein-ligand interactions in the context of intra-and intercellular signalling
  • Proteoglycans: the role of glycosaminoglycans in (patho)physiological processes
  • Protein folding: dependence on natural/synthetic ligand interactions
  • Identification of novel pharmaceutical target proteins by proteomics

 

Research Grants:

  • ¥¥ Investigation of the Biophysical and Structural Basis of the Chemokine/Heparan Sulphate Interaction ¥¥ funded by the Austrian Science Fund
  • ¥¥ The Role of Heparan Sulphate in Chemokine-Mediated Neoplastic Angiogenesis ¥¥ funded by the Oesterreichischen Nationalbank
  • ¥¥ A Proteomics and Structural Genomics Approach to Therapeutic Targeting Glycosaminoglycan-Binding Proteins ¥¥ funded by the European Commission

 

Teaching:

Lectures:

Mechanik & Thermodynamik

Elektrizität und Magnetismus

Geometrische Optik

Introduction to Physical Methods in Organic Chemistry

Pharmaceutical Bio- and Gentechnology

Molecular Basis of Modern Drug Development

Introduction into Methods of Biological Physics

Methods of Biological Physics

Proteomics (at the University of Vienna)

Glykobiophysics (at the University of Vienna)

Laboratory Courses:

Practical Course in Pharmaceutical Chemistry (II)

Practical Course in Pharmaceutical Chemistry (III)

Methods in Chemical Diagnostics

Instrumental Analytical Methods in Pharmacy

 

Scientific Organisations
  • Chairman of the Working Group Biochemistry of the Austrian Chemical Society
  • ICMSB: Head of the organising committee of the biannual International Conference on Molecular Structural Biology ICMSB (http://pharmchem.kfunigraz.ac.at/icmsb/) which features sessions on Novel Structures, Protein Folding, Rational Drug Design, Protein Structure Prediction and Simulation, and Structural Genomics. The next ICMSB will take place in Vienna in September 2003

    •  

    Collaborations:

     

    Scientific Career:

    1988-1991: PhD-thesis on the photophysics and molecular dynamics computer simulations of proteins (University of Vienna)

    1991-1994: Postdoctoral studies on the crystallisation and the crystallography of proteins (Max-Planck-Institut, Martinsried)

    1994-1995: Postdoctoral fellow of the Sandoz Research Institute (Vienna), investigations of drug receptor interactions

    1995-1997: APART fellowship (Austrian Academy of Sciences) on the structure, dynamics, and function of proteins interacting with drugs

    1997: Assistant Professor at the University of Graz, Institute of Pharmaceutical Chemistry

    1999: Habilitation in Biophysical Chemistry (University of Vienna); Associate Professor (Ao. Univ.-Prof.) at the University of Graz

    2001: Coordinator of the EU project ¥¥GAG Proteome Targeting¥¥

     

    Selected Publications:

    B. Goger, Y. Halden, A. Rek, R. M–sl, D. Pye, J. Gallagher, A.J. Kungl. Different affinities of glycosaminoglycan oligosaccharides for monomeric and dimeric interleukin-8. Submitted to Biochemistry

    E. Krieger, B. Goger, E. Geretti, A.J. Kungl. A structural model for the interaction of interleukin-8 and glycosaminoglycans: evidence for a disaccharide binding motif. Submitted to Protein Science

    S. F. Falsone, M. Weichel, R. Crameri, M. Breitenbach, A. J. Kungl. Oligomerisation and double stranded DNA binding of the cold shock protein homologue Cla h 8 from Cladosporium herbarum. Submitted to J. Biol. Chem.

    B. Simon-Nobbe, R. Kodzius, A. Kajava, F. Ferreira, A. Kungl, G. Achatz, R. Crameri, C. Ebner, M. Breitenbach. Structure of an IgE-Binding Peptide from Fungal Enolases. Int Arch Allergy Immunol. 124, 93-4 (2001).

    A.C Gorren, A.J. Kungl, K. Schmidt, E.R. Werner, B. Mayer. Electrochemistry of pterin cofactors and inhibitors of nitric oxide synthase. Nitric Oxide 5, 176-86 (2001).

    S. Kristl, S. Zhao, S.F. Falsone, R.L. Somerville, A.J. Kungl. The influence of ATP on the association and unfolding of the tyrosine repressor ligand response domain of Haemophilus influenzae. Biochem Biophys Res Commun. 280, 81-4 (2001).

    S.F. Falsone, R. Kurkela, G. Chiarandini, P. Vihko, A.J. Kungl. Ligand affinity, homodimerisation and ligand-induced secondary structural change and of the human vitamin D receptor. Biochem. Biophys. Res. Commun. 285, 1180-1185 (2001).

    S. Kristl, S. Zhao, B. Knappe, R.L. Somerville, A.J. Kungl. The Influence of ATP on the Binding of Aromatic Amino Acids to the Ligand Response Domain of the Tyrosine Repressor of H. influenzae. FEBS Letters 467, 87-90 (2000).

    S. Ringhofer, J. Kallen, R. Dutzler, A. Billich, A.J.W.G. Visser, D. Scholz, O. Steinhauser, H. Schreiber, M. Auer, A.J. Kungl. X-Ray Structure and Conformational Dynamics of the HIV-1 Protease in Complex with the Inhibitor SDZ283-910: Agreement of Time-Resolved Spectroscopy and Molecular Dynamics Simulations. J. Mol. Biol. 286, 1147-1160 (1999)

    A.J. Kungl, N.V. Visser, A. van Hoek, A.J.W.G. Visser, A. Billich, A. Schilk, H. Gstach, M. Auer. Time-Resolved Anisotropy of HIV-1 Protease Inhibitor Complexes Correlates with Inhibitory Activity. Biochemistry 37: 2778-2786 (1998).

    F. Ferreira, C. Ebner, B. Kramer, G. Casari, P. Briza, A.J. Kungl, R. Grimm, B. Jahn-Schmid, H. Breiteneder, D. Kraft, M. Breitenbach, H.-J. Rheinberger, O. Scheiner. Modulation of IgE Reactivity of Allergens by Site-Directed Mutagenesis: Potential Use of Hypoallergenic Variants for Immunotherapy. FASEB J. 12:231-242 (1998).

    E. Engel, K. Richter, G. Obermeyer, P. Briza, A.J. Kungl, B. Simon, M. Auer, C. Ebner, H.-J. Rheinberger, M. Breitenbach, F. Ferreira. Immunological and Biological Properties of Bet v 4, a Novel Birch Pollen Allergen with Two EF-Hand Calcium-Binding Domains. J. Biol. Chem. 272: 28630-28637 (1997).

    P. J. Andrew, M. Auer, I. J. D. Lindley, H. F. Kauffmann, A. J. Kungl. A Novel Method for Detecting Nitric Oxide in Biological Samples by TimeResolved Fluorescence Spectroscopy. FEBS Letters 408: 319-323 (1997).





    Sites of Interest:





    Further information: